{"id":2071,"date":"2026-11-03T19:22:47","date_gmt":"2026-11-03T18:22:47","guid":{"rendered":"https:\/\/doktorpeptid.sk\/cistota-peptidu-99-percent-hplc\/"},"modified":"2026-11-03T19:22:47","modified_gmt":"2026-11-03T18:22:47","slug":"cistota-peptidu-99-percent-hplc","status":"publish","type":"post","link":"https:\/\/doktorpeptid.sk\/en\/cistota-peptidu-99-percent-hplc\/","title":{"rendered":"\u010cistota peptidu 99 %: \u010do to \u010d\u00edslo znamen\u00e1 a \u010do v\u00e1m nepovie"},"content":{"rendered":"<style>\n.dp-lead{font-size:1.15em;line-height:1.7;color:#1b2440}\n.dp-note{background:#f3f6ff;border:1px solid #d9e2ff;border-left:4px solid #1f5bff;border-radius:12px;padding:16px 20px;margin:24px 0;font-size:.95em;color:#24304f}\n.dp-note--end{border-left-color:#ff4d5e;background:#fff5f6;border-color:#ffd9de}\n.dp-table-wrap{overflow-x:auto;margin:20px 0 28px;border:1px solid #e3e8f2;border-radius:14px}\n.dp-table{width:100%;border-collapse:collapse;font-size:.95em;margin:0}\n.dp-table th{background:#0b1530;color:#fff;text-align:left;padding:12px 14px;font-weight:700}\n.dp-table th:nth-child(2){color:#7fd6ff}.dp-table th:nth-child(3){color:#ffc56b}\n.dp-table td{padding:11px 14px;border-top:1px solid #e9edf5;vertical-align:top}\n.dp-table td:first-child{font-weight:700;color:#1b2440;white-space:nowrap;background:#f8faff}\n.dp-fig{margin:28px auto;text-align:center}\n.dp-fig img{border-radius:16px;max-width:100%;height:auto;box-shadow:0 12px 40px rgba(11,21,48,.18)}\n.dp-fig--formula img{background:#fff;padding:18px;box-shadow:none;border:1px solid #e3e8f2;max-height:420px;width:auto}\n.dp-fig figcaption{font-size:.85em;color:#6b7690;margin-top:10px}\n.dp-cards{display:grid;grid-template-columns:repeat(auto-fit,minmax(220px,1fr));gap:16px;margin:20px 0}\n.dp-card{border-radius:16px;padding:20px 22px;color:#fff}\n.dp-card p{margin:8px 0 0;color:rgba(255,255,255,.88);font-size:.95em}\n.dp-card--blue{background:linear-gradient(135deg,#0b2a6b,#1f5bff)}.dp-card--gold{background:linear-gradient(135deg,#5a3200,#d98a12)}\n.dp-card__k{font-size:.8em;letter-spacing:.12em;text-transform:uppercase;opacity:.8}.dp-card__t{font-size:1.35em;font-weight:800;margin-top:2px}\n.dp-product{display:grid;grid-template-columns:repeat(auto-fit,minmax(180px,1fr));gap:12px;margin:22px 0}\n.dp-product__card{display:flex;flex-direction:column;gap:4px;padding:16px 18px;border:1px solid #dfe5f1;border-radius:14px;text-decoration:none!important;color:#0b1530!important;transition:.2s}\n.dp-product__card:hover{border-color:#1f5bff;box-shadow:0 8px 24px rgba(31,91,255,.15);transform:translateY(-2px)}\n.dp-product__k{font-size:.75em;text-transform:uppercase;letter-spacing:.1em;color:#1f5bff;font-weight:700}.dp-product__t{font-weight:800}.dp-product__s{font-size:.85em;color:#6b7690}\n.dp-sources{font-size:.88em;color:#46506a}.dp-sources li{margin-bottom:6px}\n.dp-product br,.dp-cards br{display:none}\n<\/style>\n<p class=\"dp-lead\">\u201e\u010cistota 99 %.\u201c Toto \u010d\u00edslo vid\u00edte pri peptidoch takmer v\u0161ade. \u013dahko z neho vyvod\u00edme, \u017ee 99 % pr\u00e1\u0161ku vo f\u013ea\u0161ti\u010dke je samotn\u00fd peptid. V skuto\u010dnosti v\u0161ak znamen\u00e1 nie\u010do in\u00e9. V tomto \u010dl\u00e1nku vysvet\u013eujeme, odkia\u013e \u010d\u00edslo 99 % poch\u00e1dza, \u010do z neho vy\u010d\u00edta\u0165 ned\u00e1 a pre\u010do poriadny analytick\u00fd certifik\u00e1t uv\u00e1dza viac \u00fadajov ako jedno percento.<\/p>\n<h2>Odkia\u013e sa berie \u201e99 %\u201c<\/h2>\n<p>\u010cistota peptidov sa takmer v\u017edy meria met\u00f3dou <strong>HPLC<\/strong> (vysoko\u00fa\u010dinn\u00e1 kvapalinov\u00e1 chromatografia). Z\u00e1kladn\u00fa my\u0161lienku chromatografie op\u00edsal na za\u010diatku 20. storo\u010dia rusk\u00fd botanik Michail Cvet, ke\u010f na st\u013apci uhli\u010ditanu v\u00e1penat\u00e9ho rozdelil rastlinn\u00e9 farbiv\u00e1 na farebn\u00e9 p\u00e1sy. Odtia\u013e poch\u00e1dza aj n\u00e1zov: <em>chroma<\/em> znamen\u00e1 farba. Modern\u00e9 HPLC pr\u00edstroje, ktor\u00e9 pracuj\u00fa pod vysok\u00fdm tlakom s ve\u013emi jemn\u00fdmi \u010dasticami v kol\u00f3ne, sa roz\u0161\u00edrili v 60. a 70. rokoch.<\/p>\n<p>Princ\u00edp je jednoduch\u00fd. Rozpusten\u00e1 vzorka prete\u010die kol\u00f3nou a ka\u017ed\u00e1 l\u00e1tka v nej putuje inou r\u00fdchlos\u0165ou. Na konci kol\u00f3ny ich zachyt\u00ed detektor. Pri peptidoch je to naj\u010dastej\u0161ie UV detektor nastaven\u00fd okolo 214 a\u017e 220 nm, kde pohlcuje svetlo peptidov\u00e1 v\u00e4zba. V\u00fdsledkom je <strong>chromatogram<\/strong>: krivka s vrcholmi, kde ka\u017ed\u00fd vrchol zodpoved\u00e1 jednej l\u00e1tke.<\/p>\n<figure class=\"dp-fig\"><img decoding=\"async\" class=\"wp-image-2066\" src=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-1024x1024.jpg\" alt=\"Kol\u00f3na HPLC a chromatogram s hlavn\u00fdm vrcholom \u2013 ilustr\u00e1cia\" width=\"1024\" height=\"1024\" loading=\"lazy\"  title=\"\u010cistota peptidu 99 %: \u010do to \u010d\u00edslo znamen\u00e1 a \u010do v\u00e1m nepovie\" srcset=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-1024x1024.jpg 1024w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-300x300.jpg 300w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-150x150.jpg 150w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-768x768.jpg 768w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-12x12.jpg 12w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-700x700.jpg 700w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-600x600.jpg 600w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia-100x100.jpg 100w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hplc-chromatogram-ilustracia.jpg 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption>Ilustr\u00e1cia z videa DoktorPeptid: l\u00e1tky sa v kol\u00f3ne rozdelia a detektor ich zap\u00ed\u0161e ako vrcholy chromatogramu.<\/figcaption><\/figure>\n<p>\u201e\u010cistota 99 %\u201c potom znamen\u00e1, \u017ee <strong>plocha hlavn\u00e9ho vrcholu tvor\u00ed 99 % plochy v\u0161etk\u00fdch vrcholov<\/strong>, ktor\u00e9 detektor zaznamenal. Je to teda relat\u00edvny \u00fadaj. Porovn\u00e1va peptid s ne\u010distotami, ktor\u00e9 detektor \u201evid\u00ed\u201c, napr\u00edklad so skr\u00e1ten\u00fdmi alebo po\u0161koden\u00fdmi re\u0165azcami zo synt\u00e9zy.<\/p>\n<h2>Prv\u00e1 vec, ktor\u00fa HPLC nepovie: \u010di je to ten spr\u00e1vny peptid<\/h2>\n<p>Chromatogram uk\u00e1\u017ee, \u017ee vo vzorke je jedna dominantn\u00e1 l\u00e1tka. S\u00e1m osebe v\u0161ak nepovie, <strong>ak\u00e1<\/strong> l\u00e1tka to je. Vrchol prezrad\u00ed len \u010das, za ktor\u00fd l\u00e1tka pre\u0161la kol\u00f3nou, a ten m\u00f4\u017ee by\u0165 pri dvoch podobn\u00fdch molekul\u00e1ch takmer rovnak\u00fd.<\/p>\n<p>Na identitu sa pou\u017e\u00edva <strong>hmotnostn\u00e1 spektrometria (MS)<\/strong>. T\u00e1 molekuly zjednodu\u0161ene povedan\u00e9 odv\u00e1\u017ei: premen\u00ed ich na i\u00f3ny a zmeria pomer ich hmotnosti a n\u00e1boja. Ak nameran\u00e1 hmotnos\u0165 sed\u00ed s teoretickou hmotnos\u0165ou dan\u00e9ho peptidu, m\u00e1me potvrden\u00fa identitu. Napr\u00edklad BPC-157 (sum\u00e1rny vzorec C<sub>62<\/sub>H<sub>98<\/sub>N<sub>16<\/sub>O<sub>22<\/sub>) m\u00e1 mol\u00e1rnu hmotnos\u0165 pribli\u017ene 1419,5 g\/mol.<\/p>\n<figure class=\"dp-fig\"><img decoding=\"async\" class=\"wp-image-2067\" src=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-1024x1024.jpg\" alt=\"Hmotnostn\u00e1 spektrometria overuje identitu peptidu \u2013 ilustr\u00e1cia\" width=\"1024\" height=\"1024\" loading=\"lazy\"  title=\"\u010cistota peptidu 99 %: \u010do to \u010d\u00edslo znamen\u00e1 a \u010do v\u00e1m nepovie\" srcset=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-1024x1024.jpg 1024w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-300x300.jpg 300w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-150x150.jpg 150w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-768x768.jpg 768w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-12x12.jpg 12w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-700x700.jpg 700w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-600x600.jpg 600w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia-100x100.jpg 100w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/hmotnostna-spektrometria-ilustracia.jpg 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption>Ilustr\u00e1cia: hmotnostn\u00e1 spektrometria overuje identitu tak, \u017ee molekulu \u201eodv\u00e1\u017ei\u201c.<\/figcaption><\/figure>\n<p>Prv\u00e9 hmotnostn\u00e9 spektrometre zostrojil u\u017e za\u010diatkom 20. storo\u010dia J. J. Thomson. Pri ve\u013ek\u00fdch a krehk\u00fdch biomolekul\u00e1ch, ak\u00fdmi s\u00fa peptidy a bielkoviny, v\u0161ak met\u00f3da dlho nar\u00e1\u017eala na probl\u00e9m, ako ich dosta\u0165 do plynnej f\u00e1zy bez rozpadu. Zlom priniesli techniky <strong>ESI<\/strong> (John B. Fenn) a <strong>MALDI<\/strong> (Koichi Tanaka), za ktor\u00e9 v roku 2002 udelili Nobelovu cenu za ch\u00e9miu. Dnes je spojenie HPLC a MS (LC-MS) \u0161tandardom v anal\u00fdze peptidov.<\/p>\n<h2>Druh\u00e1 vec: pr\u00e1\u0161ok nie je len peptid<\/h2>\n<p>Syntetick\u00e9 peptidy sa dnes vyr\u00e1baj\u00fa takmer v\u00fdhradne synt\u00e9zou na pevnej f\u00e1ze (SPPS), ktor\u00fa v 60. rokoch vyvinul Robert Bruce Merrifield. Za \u0148u dostal v roku 1984 Nobelovu cenu. Pri od\u0161tiepen\u00ed hotov\u00e9ho re\u0165azca z nosi\u010da aj pri \u010disten\u00ed na HPLC sa be\u017ene pou\u017e\u00edva <strong>kyselina triflu\u00f3roctov\u00e1 (TFA)<\/strong>. Kladne nabit\u00e9 skupiny peptidu na seba potom via\u017eu jej ani\u00f3ny. Peptid tak vznik\u00e1 ako <strong>so\u013e<\/strong>, nie ako \u201ehol\u00e1\u201c molekula.<\/p>\n<p>Lyofilizovan\u00fd pr\u00e1\u0161ok navy\u0161e \u013eahko via\u017ee vzdu\u0161n\u00fa vlhkos\u0165, tak\u017ee obsahuje aj mal\u00e9 mno\u017estvo vody. Soli aj voda maj\u00fa svoju hmotnos\u0165. Do \u010distoty z HPLC sa v\u0161ak nezapo\u010d\u00edtaj\u00fa, lebo ich UV detektor pri hlavnom vrchole nezachyt\u00ed.<\/p>\n<figure class=\"dp-fig\"><img decoding=\"async\" class=\"wp-image-2068\" src=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-1024x1024.jpg\" alt=\"Pr\u00e1\u0161ok tvor\u00ed peptid, soli TFA a voda \u2013 ilustr\u00e1cia\" width=\"1024\" height=\"1024\" loading=\"lazy\"  title=\"\u010cistota peptidu 99 %: \u010do to \u010d\u00edslo znamen\u00e1 a \u010do v\u00e1m nepovie\" srcset=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-1024x1024.jpg 1024w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-300x300.jpg 300w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-150x150.jpg 150w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-768x768.jpg 768w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-12x12.jpg 12w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-700x700.jpg 700w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-600x600.jpg 600w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia-100x100.jpg 100w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/peptid-soli-voda-ilustracia.jpg 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption>Ilustr\u00e1cia: pr\u00e1\u0161ok vo f\u013ea\u0161ti\u010dke tvor\u00ed peptid (modr\u00fd), protii\u00f3ny sol\u00ed (\u017elt\u00e9) a voda (tmavomodr\u00e1). \u010cistota z HPLC hodnot\u00ed len peptidov\u00fa \u010das\u0165.<\/figcaption><\/figure>\n<p>Dobre to ukazuje \u0161t\u00fadia z roku 2025 od \u0161vaj\u010diarskych v\u00fdskumn\u00edkov (Erckes a kol., <em>Pharmaceuticals<\/em>). Autori pri syntetick\u00fdch peptidoch vymenili protii\u00f3n TFA za chlorid a obsah zvy\u0161n\u00e9ho TFA merali tromi r\u00f4znymi met\u00f3dami. \u010cistota peptidov sa pri v\u00fdmene soli <strong>nezmenila pri \u017eiadnej<\/strong> z testovan\u00fdch koncentr\u00e1ci\u00ed kyseliny. In\u00fdmi slovami, percento \u010distoty ni\u010d nehovor\u00ed o tom, ak\u00e1 so\u013e a ko\u013eko jej je v pr\u00e1\u0161ku. Autori z\u00e1rove\u0148 upozor\u0148uj\u00fa, \u017ee so\u013e TFA m\u00f4\u017ee skresli\u0165 nava\u017eovanie, a odpor\u00fa\u010daj\u00fa obsah protii\u00f3nov v\u017edy mera\u0165 a uv\u00e1dza\u0165.<\/p>\n<h2>\u010cistota, identita a obsah: tri r\u00f4zne ot\u00e1zky<\/h2>\n<div class=\"dp-table-wrap\">\n<table class=\"dp-table\">\n<thead>\n<tr>\n<th>\u00dadaj<\/th>\n<th>Na \u010do odpoved\u00e1<\/th>\n<th>Typick\u00e1 met\u00f3da<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Cleanliness<\/td>\n<td>Ak\u00fd podiel tvor\u00ed hlavn\u00e1 l\u00e1tka medzi zachyten\u00fdmi l\u00e1tkami?<\/td>\n<td>HPLC s UV detektorom<\/td>\n<\/tr>\n<tr>\n<td>Identita<\/td>\n<td>Je hlavn\u00e1 l\u00e1tka naozaj deklarovan\u00fd peptid?<\/td>\n<td>hmotnostn\u00e1 spektrometria (MS, LC-MS)<\/td>\n<\/tr>\n<tr>\n<td>Obsah (mno\u017estvo)<\/td>\n<td>Ko\u013eko miligramov peptidu je vo f\u013ea\u0161ti\u010dke?<\/td>\n<td>kvantifik\u00e1cia oproti \u0161tandardu, aminokyselinov\u00e1 anal\u00fdza<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p>Vysok\u00e1 \u010distota je teda dobr\u00e1 spr\u00e1va, no odpoved\u00e1 len na prv\u00fa ot\u00e1zku. \u00dapln\u00fd obraz o vzorke d\u00e1vaj\u00fa a\u017e v\u0161etky tri \u00fadaje spolu.<\/p>\n<h2>\u010co by mal obsahova\u0165 poriadny certifik\u00e1t<\/h2>\n<p>Analytick\u00fd certifik\u00e1t (COA) by mal by\u0165 \u010ditate\u013en\u00fd aj pre laika. Okrem percenta \u010distoty m\u00e1 zmysel h\u013eada\u0165 na \u0148om:<\/p>\n<ul>\n<li>n\u00e1zov nez\u00e1visl\u00e9ho laborat\u00f3ria a d\u00e1tum anal\u00fdzy,<\/li>\n<li>identifik\u00e1ciu vzorky alebo \u0161ar\u017ee,<\/li>\n<li>potvrdenie identity, typicky hmotnostnou spektrometriou,<\/li>\n<li>nameran\u00fd obsah peptidu v miligramoch, nielen percent\u00e1,<\/li>\n<li>mo\u017enos\u0165 overi\u0165 pravos\u0165 dokumentu, napr\u00edklad k\u00f3dom na webe laborat\u00f3ria.<\/li>\n<\/ul>\n<figure class=\"dp-fig\"><img decoding=\"async\" class=\"wp-image-2069\" src=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-1024x1024.jpg\" alt=\"Certifik\u00e1t: \u010distota, identita a obsah peptidu \u2013 ilustr\u00e1cia\" width=\"1024\" height=\"1024\" loading=\"lazy\"  title=\"\u010cistota peptidu 99 %: \u010do to \u010d\u00edslo znamen\u00e1 a \u010do v\u00e1m nepovie\" srcset=\"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-1024x1024.jpg 1024w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-300x300.jpg 300w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-150x150.jpg 150w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-768x768.jpg 768w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-12x12.jpg 12w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-700x700.jpg 700w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-600x600.jpg 600w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia-100x100.jpg 100w, https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/coa-cistota-identita-obsah-ilustracia.jpg 1080w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption>Ilustr\u00e1cia: tri \u00fadaje, ktor\u00e9 spolu d\u00e1vaj\u00fa zmysel \u2013 \u010distota, identita a obsah.<\/figcaption><\/figure>\n<p>Certifik\u00e1ty k na\u0161im \u0161ar\u017eiam z nez\u00e1visl\u00e9ho laborat\u00f3ria n\u00e1jdete na str\u00e1nke <a href=\"\/en\/coa\/\">COA certifik\u00e1ty<\/a>. Okrem \u010distoty uv\u00e1dzaj\u00fa aj nameran\u00e9 mno\u017estvo peptidu a k\u00f3d na overenie.<\/p>\n<div class=\"dp-product\"><a class=\"dp-product__card\" href=\"\/en\/coa\/\"><span class=\"dp-product__k\">Documents<\/span><span class=\"dp-product__t\">COA certifik\u00e1ty<\/span><span class=\"dp-product__s\">\u010distota aj obsah \u0161ar\u017e\u00ed<\/span><\/a><a class=\"dp-product__card\" href=\"\/en\/ako-skladovat-lyofilizovane-peptidy\/\"><span class=\"dp-product__k\">\u010cl\u00e1nok<\/span><span class=\"dp-product__t\">Lyofilizovan\u00e9 peptidy<\/span><span class=\"dp-product__s\">\u010do je lyofiliz\u00e1t<\/span><\/a><a class=\"dp-product__card\" href=\"\/en\/category\/blog\/\"><span class=\"dp-product__k\">Blog<\/span><span class=\"dp-product__t\">V\u0161etky \u010dl\u00e1nky<\/span><span class=\"dp-product__s\">peptidy vo v\u00fdskume<\/span><\/a><\/div>\n<h2>Frequently asked questions<\/h2>\n<h3>Znamen\u00e1 \u010distota 99 %, \u017ee 99 % pr\u00e1\u0161ku je peptid?<\/h3>\n<p>Nie. \u010cistota z HPLC hovor\u00ed, ak\u00fd podiel tvor\u00ed hlavn\u00fd vrchol medzi l\u00e1tkami, ktor\u00e9 zachytil detektor. Soli (napr\u00edklad TFA) a voda sa do nej nezapo\u010d\u00edtavaj\u00fa, hoci tvoria \u010das\u0165 hmotnosti pr\u00e1\u0161ku.<\/p>\n<h3>Pre\u010do nesta\u010d\u00ed samotn\u00e9 HPLC?<\/h3>\n<p>HPLC uk\u00e1\u017ee, ako \u010dist\u00e1 je vzorka, ale nepotvrd\u00ed, o ak\u00fa molekulu ide. Identitu overuje hmotnostn\u00e1 spektrometria, ktor\u00e1 zmeria hmotnos\u0165 molekuly.<\/p>\n<h3>\u010co je TFA a pre\u010do je v peptidoch?<\/h3>\n<p>Kyselina triflu\u00f3roctov\u00e1 sa pou\u017e\u00edva pri synt\u00e9ze a \u010disten\u00ed peptidov. Jej ani\u00f3ny sa via\u017eu na kladne nabit\u00e9 skupiny peptidu, tak\u017ee peptid vznik\u00e1 vo forme soli.<\/p>\n<h2>Zdroje<\/h2>\n<ol class=\"dp-sources\">\n<li>Erckes V., Streuli A., Chamera Rendueles L., Kr\u00e4mer S. D., Steuer C. Towards a Consensus for the Analysis and Exchange of TFA as a Counterion in Synthetic Peptides and Its Influence on Membrane Permeation. <em>Pharmaceuticals<\/em>, 2025. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40872554\/\" target=\"_blank\" rel=\"noopener\">PubMed 40872554<\/a><\/li>\n<li>Merrifield R. B. Solid Phase Peptide Synthesis. I. The Synthesis of a Tetrapeptide. <em>J. Am. Chem. Soc.<\/em>, 1963. <a href=\"https:\/\/doi.org\/10.1021\/ja00897a025\" target=\"_blank\" rel=\"noopener\">doi:10.1021\/ja00897a025<\/a><\/li>\n<li>Nobelova cena za ch\u00e9miu 2002 (Fenn, Tanaka, W\u00fcthrich). <a href=\"https:\/\/www.nobelprize.org\/prizes\/chemistry\/2002\/summary\/\" target=\"_blank\" rel=\"noopener\">nobelprize.org<\/a><\/li>\n<li>Wikipedia: <a href=\"https:\/\/en.wikipedia.org\/wiki\/High-performance_liquid_chromatography\" target=\"_blank\" rel=\"noopener\">High-performance liquid chromatography<\/a>, <a href=\"https:\/\/en.wikipedia.org\/wiki\/Mass_spectrometry\" target=\"_blank\" rel=\"noopener\">Mass spectrometry<\/a>, <a href=\"https:\/\/en.wikipedia.org\/wiki\/Peptide_synthesis\" target=\"_blank\" rel=\"noopener\">Peptide synthesis<\/a><\/li>\n<\/ol>\n<div class=\"dp-note dp-note--end\">\u010cl\u00e1nok m\u00e1 eduka\u010dn\u00fd charakter. Produkty DoktorPeptid s\u00fa ur\u010den\u00e9 v\u00fdhradne na vedeck\u00e9 a laborat\u00f3rne \u00fa\u010dely.<\/div>","protected":false},"excerpt":{"rendered":"<p>\u010cistota 99 % neznamen\u00e1, \u017ee 99 % pr\u00e1\u0161ku je peptid. Vysvet\u013eujeme, \u010do meria HPLC, pre\u010do identitu potvrdzuje hmotnostn\u00e1 spektrometria a pre\u010do do \u010distoty nevstupuj\u00fa soli TFA ani voda.<\/p>","protected":false},"author":1,"featured_media":2070,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[100],"tags":[],"class_list":["post-2071","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-kvalita-a-certifikaty"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/doktorpeptid.sk\/wp-content\/uploads\/2026\/11\/cistota-peptidu-hplc-doktorpeptid.jpg","_links":{"self":[{"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/posts\/2071","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/comments?post=2071"}],"version-history":[{"count":0,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/posts\/2071\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/media\/2070"}],"wp:attachment":[{"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/media?parent=2071"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/categories?post=2071"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/doktorpeptid.sk\/en\/wp-json\/wp\/v2\/tags?post=2071"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}